ANALYSIS OF FILAMIN AND ALPHA-ACTININ BINDING TO ACTIN BY THE STOPPED-FLOW METHOD

Authors
GOLDMANN WH ISENBERG G
Citation
Wh. Goldmann et G. Isenberg, ANALYSIS OF FILAMIN AND ALPHA-ACTININ BINDING TO ACTIN BY THE STOPPED-FLOW METHOD, FEBS letters, 336(3), 1993, pp. 408-410
Citations number
16
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
336
Issue
3
Year of publication
1993
Pages
408 - 410
Database
ISI
SICI code
0014-5793(1993)336:3<408:AOFAAB>2.0.ZU;2-U
Abstract
We ascertained by the stopped flow method the overall association rate constant, k(+1), of filamin and alpha-actinin to fluorescently labell ed filamentous actin of similar to 1.3x10(6)M(-1).s(-1) and similar to 1.0x10(6)M(-1).s(-1) as well as the overall dissociation rate constan t, k(-1) of similar to 0.6s(-1) and similar to 0.4s(-1), respeetively. The overall equilibrium constant, K, for filamin and alpha-actinin to actin deduced from the relation K=k(+1)/k(-1) agree well with publish ed data.