Wh. Goldmann et G. Isenberg, ANALYSIS OF FILAMIN AND ALPHA-ACTININ BINDING TO ACTIN BY THE STOPPED-FLOW METHOD, FEBS letters, 336(3), 1993, pp. 408-410
We ascertained by the stopped flow method the overall association rate
constant, k(+1), of filamin and alpha-actinin to fluorescently labell
ed filamentous actin of similar to 1.3x10(6)M(-1).s(-1) and similar to
1.0x10(6)M(-1).s(-1) as well as the overall dissociation rate constan
t, k(-1) of similar to 0.6s(-1) and similar to 0.4s(-1), respeetively.
The overall equilibrium constant, K, for filamin and alpha-actinin to
actin deduced from the relation K=k(+1)/k(-1) agree well with publish
ed data.