ANALYSIS OF THE FEEDING MOTOR PATTERN IN THE POND SNAIL, LYMNAEA-STAGNALIS - PHOTOINACTIVATION OF AXONALLY STAINED PATTERN-GENERATING INTERNEURONS

We have photoinactivated identified feeding interneurons known as N1 a nd N2 neurons. These are pattern-generating neurons that are active in the protraction of the radula and rasping phases, respectively, of th e feeding cycle of the pond snail. The N1 or N2 feeding interneurons i n the buccal ganglia were filled with the fluorescent dye 5(6)-carboxy fluorescein (5-CF) from the cut end of the nerve that contains their a xon. Filling the cerebrobuccal connective (N = 151) stained just one N 1 cell in the contralateral buccal ganglion. Filling the postbuccal ne rve stained neurons symmetrically in both buccal ganglia (N = 75): onl y one labeled cell in each ganglion is an N2 interneuron. The feeding rhythm was evoked by depolarizing a modulatory neuron, the SO, located in the buccal ganglia. The axonally filled N1 interneuron was irradia ted at its axon in the buccal commissure with blue laser light (intens ity of 0.5 MW.m(-2)). Irradiation of just one NI completely blocked th e feeding rhythm (seven preparations). In seven further preparations, N1 ablation slowed the SO-driven feeding rhythm and weakened the N1 in put to the feeding neurons. Irradiation of the cell bodies of both the filled left and right N2 interneurons killed the cells but did not pr oduce any consistent change in the feeding rate (15 preparations). The feeding interneurons and motoneurons still showed the characteristic N2 phase synaptic inputs, so more, as yet unidentified, N2 neurons mus t be located in other parts of the buccal ganglia. We conclude that th e participation of the identified N1 interneurons is essential for the normal feeding pattern while other, still to be identified N2 neurons must be present and must contribute to the feeding rhythm. We suggest that the extra redundancy of the N2 network may be related to the gre ater necessity of sensory feedback control during rasping than during protraction of the radula.