The recently published sequence of yeast chromosome III (YCIII) provid
es the longest continuous stretch of a eukaryotic DNA molecule sequenc
ed to date (315 kb). The sequence contains 116 distinct AUG-initiated
open reading frames of at least 200 codons in length, more than 50 of
which had not been described previously nor bear significant similarit
y to known proteins. We have analysed the YCIII known and putative pro
tein sequences with respect to significant statistical features which
might reflect on structural and functional characteristics. The YCIII
proteins have striking similarities and differences in their sequence
attribute distributions compared to the corresponding distributions fo
r all available yeast sequences and other protein collections. Nine ex
amples of YCIII proteins with distinctive sequence features are discus
sed in detail.