COMPARISON OF THE EFFECTS OF 2 DIETS RICH IN MONOUNSATURATED FATTY-ACIDS DIFFERING IN THEIR LINOLEIC ALPHA-LINOLENIC ACID RATIO ON PLATELET-AGGREGATION
R. Freese et al., COMPARISON OF THE EFFECTS OF 2 DIETS RICH IN MONOUNSATURATED FATTY-ACIDS DIFFERING IN THEIR LINOLEIC ALPHA-LINOLENIC ACID RATIO ON PLATELET-AGGREGATION, Thrombosis and haemostasis, 71(1), 1994, pp. 73-77
The effect of dietary linoleic/alpha-linolenic acid ratio on human pla
telet aggregation in vitro was investigated using low-erucic acid rape
seed oil and high-oleic acid sunflower oil as the major fat sources. I
n a cross-over study 20 healthy male subjects, average age 29 year (ra
nge 20-46 yr), followed experimental rapeseed oil (RO) and Trisun-sunf
lower oil (TSO) diets after their habitual diet for six weeks. Subject
s were provided most of the fat containing foods but were allowed to e
at other foods almost freely. Fatty acid compositions of the diets cal
culated from dietary records were as follows (saturated/monounsaturate
d/polyunsaturated fatty acids): RO diet 12.4/18.6/8.9% of total energy
(cn%) (linoleic/alpha-linolenic acid ratio 2.8) and TSO diet 11.8/17.
8/8.3 en% (linoleic/alpha-linolenic acid ratio 28), respectively. Plas
ma cholesterol ester fatty acid composition proved compliance to the e
xperimental diets. Platelet aggregations induced by ADP (1, 2) and 3 m
uM) or thrombin (0. 12, 0.15 and 0. 18 NIH/ml) were significantly enha
nced and collagen- (1.5, 2.5 and 5.0 mug/ml) induced aggregation tende
d to be enhanced after the TSO dict compared with the RO dict. After t
he TSO diet platelet aggregation was enhanced from the level of the ha
bitual diets by one thrombin (0.18 NIH/ml), one collagen (1.5 mug/ml)
and all three ADP concentrations. The diets had no effect on antithrom
bin III activity. Results show that platelet aggregation in vitro decr
eases as the ratio of linoleic acid to alpha-linolenic acid decreases
in diets rich in monounsaturated fatty acids.