A PARTIAL CONNECTIN CDNA-ENCODING A NOVEL TYPE OF RSP MOTIFS ISOLATEDFROM CHICKEN EMBRYONIC SKELETAL-MUSCLE

A partial cDNA encoding 811 amino acids of connectin (titin), a giant elastic protein of muscle (3,000 kDa), was cloned from a chicken embry onic skeletal muscle cDNA library using antibodies to muscle connectin . The encoded product was the C terminal segment of connectin. The pre dicted sequences consisted of 5 type II motifs (immunoglobulin C2 type ) separated by 5 interdomain insertions. One interdomain insertion had significant homology (RSP) to KSP repeats found in human cardiac C-te rminal connectin and another had a high sequence homology to porin (67 .7%; 31 amino acids).