SITEVIDEO - A COMPUTER-SYSTEM FOR FUNCTIONAL SITE ANALYSIS AND RECOGNITION - INVESTIGATION OF THE HUMAN SPLICE SITES

We developed the computer system SITEVIDEO for analysis and recognitio n of the functional sites in DNA and RNA molecules. It reveals context ual features essential for site function and thus enable the user to d esign efficient methods for recognition of the functional sites. We ma inly considered only quantitative characteristics reflecting the uneve n distribution of oligonucleotides in the sequences of functional site s of interest. The approach suggested makes use of available informati on about the hierarchical organization of the functional sites, and en sures highly precise prediction of the sites. The present analysis is concerned with the human donor and acceptor splice sites. A method for recognizing these sites in the sequences with an accuracy of similar to 90% was developed.