The effects of raised temperature and extended photoperiod on the deha
rdening of quiescent and winter-hardy Scots pine saplings were examine
d in an open-top-chamber experiment. The saplings were exposed during
winter to natural, square-curve fluctuating (between 1 and 11 degrees
C with a 14 d interval), and constant (6 degrees C) temperatures with
a natural and an extended (17 h) photoperiod. Frost hardiness of needl
es was determined by controlled freezing tests and visual damage scori
ng. The constant 6 degrees C temperature treatment caused a gradual de
hardening of needles whereas under fluctuating temperatures the level
of frost hardiness fluctuated. Trees exposed to extended photoperiods
were less hardy than under natural photoperiods after the initiation o
f shoot elongation, but before this there were no clear differences in
frost hardiness between different photoperiodic treatments. The resul
ts indicate that the Best hardening competence of Scots pine changes d
uring quiescence. (C) 1997 Annals of Botany Company.