DETECTION OF ERGOT (CLAVICEPS-PURPUREA) IN A DAIRY FEED COMPONENT BY GAS-CHROMATOGRAPHY AND MASS-SPECTROMETRY

A dairy feed that contained barley malt screenings caused hyperthermia in dairy cattle. The feed was suspected of containing ergot and was s ubsequently analyzed to determine the ricinoleate component, a special and prominent feature of oil-rich ergot tissue. Triglyceride oil was extracted by organic solvents from the dairy feed, and the oil was sap onified to release fatty acids. Ricinoleate, as a methyl ester, was se lectively resolved from other fatty acids by silica gel chromatography and was analyzed by capillary gas chromatography coupled with chemica l ionization mass spectrometry, which demonstrated the presence of the methyl ricinoleate molecule and proved that the feed contained ergot. The methodology may be refined to monitor for ergot in powdered dairy feed more routinely.