STRUCTURE-BASED RATIONALE FOR THE RESCUE OF SYSTEMIC MOVEMENT OF BROME MOSAIC-VIRUS BY SPONTANEOUS 2ND-SITE MUTATIONS IN THE COAT PROTEIN GENE

Authors
FLASINSKI S DZIANOTT A SPEIR JA JOHNSON JE BUJARSKI JJ
Citation
S. Flasinski et al., STRUCTURE-BASED RATIONALE FOR THE RESCUE OF SYSTEMIC MOVEMENT OF BROME MOSAIC-VIRUS BY SPONTANEOUS 2ND-SITE MUTATIONS IN THE COAT PROTEIN GENE, Journal of virology, 71(3), 1997, pp. 2500-2504
Citations number
28
Categorie Soggetti
Virology
Journal title
Journal of virology → ACNP
ISSN journal
0022538X
Volume
71
Issue
3
Year of publication
1997
Pages
2500 - 2504
Database
ISI
SICI code
0022-538X(1997)71:3<2500:SRFTRO>2.0.ZU;2-T
Abstract
We describe spontaneous second-site reversions within the coat protein open reading frame that rescue the systemic-spread phenotype and incr ease virion stability of a mutant of brome mosaic virus. Based on the crystal structure of the related cowpea chlorotic mottle virus, we sho w that the modified residues are spatially clustered to affect the for mation of hexamers and pentamers and therefore virion stability.