DETERMINATION OF LOMEFLOXACIN IN HUMAN PLASMA BY SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH UV DETECTION

A high-performance liquid chromatographic method for the determination of lomefloxacin in human plasma has been developed and validated. A s olid-phase extraction procedure was used to isolate lomefloxacin from the biological matrix prior to the quantitative analysis. The compound was separated on a Vydac anion-exchange column using acetonitrile-pho sphate buffer (pH 7.0) as the mobile phase and quantified by measuring its UV absorbance at 280 nm. The lower limit of detection for the ana lyte was 0.05 mu g ml(-1). Enoxacin was used as the internal standard. The calibration graph of the method was linear from 0.1 to 10 mu g ml (-1) of lomefloxacin in human plasma. This procedure is suitable for p harmacological and pharmacokinetic studies of lomefloxacin.