CHIRAL HPLC VERSUS CHIRAL SFC - EVALUATION OF LONG-TERM STABILITY ANDSELECTIVITY OF CHIRALCEL OD USING VARIOUS ELUENTS

The long-term stability of Chiralcel OD columns under varying HPLC and SFC conditions was evaluated. Two new columns from the same supplier lot were procured, and one was installed in an HPLC system, and the ot her was installed in an SFC system. Enantiomeric mixtures of trans-sti lbene bride and carbobenzyloxy phenylalaninol were repeatedly injected in both systems over several days. For HPLC, hexane was the primary s olvent used, along with IPA, EtOH, MeOH/IPA, or EtOH/TFA modifier. Car bon dioxide was the primary SFC solvent, together with the similar mod ifiers as the HPLC. Column performance was monitored by measuring reso lution, theoretical plate number, and or between enantiomeric peaks. I t was observed that, when the eluent strengths were adjusted to provid e comparable retention times, the Chiralcel OD gave superior enantiome ric resolution when used in the SFC than the HPLC. Equilibration in be tween solvents was faster with SFC. The columns were demonstrated to b e guile stable in both systems