ANALYSIS OF FCE-23762 (METHOXYMORPHOLINODOXORUBICIN HYDROCHLORIDE), ANEW ANTITUMOR AGENT, BY HPTLC AND SCANNING DENSITOMETRY

Authors
FARINA A QUAGLIA MG DOLDO A CALANDRA S GALLO FR
Citation
A. Farina et al., ANALYSIS OF FCE-23762 (METHOXYMORPHOLINODOXORUBICIN HYDROCHLORIDE), ANEW ANTITUMOR AGENT, BY HPTLC AND SCANNING DENSITOMETRY, Journal of pharmaceutical and biomedical analysis, 11(11-12), 1993, pp. 1215-1218
Citations number
12
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
Journal of pharmaceutical and biomedical analysis → ACNP
ISSN journal
07317085
Volume
11
Issue
11-12
Year of publication
1993
Pages
1215 - 1218
Database
ISI
SICI code
0731-7085(1993)11:11-12<1215:AOF(HA>2.0.ZU;2-U
Abstract
A simple, rapid and reproducible high-performance thin-layer chromatog raphic (HPTLC) method using UV or fluorescence scanning densitometry h as been developed for the assay and purity control of methoxymorpholin odoxorubicin hydrochloride (FCE 23762). With a mobile phase of chlorof orm-methanol-acetic acid (93:6:1, v/v/v) and a silica gel plate, all p otential impurities were separated from the main component and from ea ch other. Detection limits at a signal to-noise ratio of 2:1 were a fe w nanograms for UV detection and <1 ng for fluorescence emission. The RSD values for the examined compounds were all <3%.