THE STRUCTURE OF THE HUMAN THYROXINE-BINDING GLOBULIN (TBG) GENE

A portion of the human X-chromosome (> 5 kb) encoding the translated p ortion of the thyroxine-binding globulin (TBG) gene was sequenced. The primary templates for sequencing were isolated from a human X-chromos ome library (two positive plaques from 400 000 screened initially with a TBG cDNA probe) or were produced by PCR amplification using leucocy te genomic DNA as the amplification template. Potential hormone respon se elements (HREs) were identified at either end of the gene. These HR Es have sequences based on the consensus half-site of thyroid hormone response elements, although it is unclear whether the structures are f unctional HREs. Other potential regulatory elements also were identifi ed towards the 3' end of the gene.