PROTON NMR LIPID PROFILE OF LEISHMANIA-DONOVANI PROMASTIGOTES

The proton nuclear magnetic resonance (NMR) lipid profile of Leishmani a donovani was obtained in the one-dimensional and two-dimensional mod es. Partial assignments of lipid classes and individual lipids were ob tained purely from the proton NMR spectrum of the mixture. A more comp lete assignment and quantitative analysis was achieved by prior separa tion of the lipids by high pressure liquid chromatography (HPLC) follo wed by proton NMR analysis of the fractions. This work showed that pro ton NMR spectroscopy could facilitate lipid analysis and classificatio n of various parasitic protozoa and serve as a basis for rapid studies of comparative lipid metabolism in parasites.