PROTON NMR LIPID PROFILE OF LEISHMANIA-DONOVANI PROMASTIGOTES

Citation
Rk. Adosraku et al., PROTON NMR LIPID PROFILE OF LEISHMANIA-DONOVANI PROMASTIGOTES, Molecular and biochemical parasitology, 62(2), 1993, pp. 251-262
Citations number
20
Categorie Soggetti
Parasitiology,Biology
ISSN journal
01666851
Volume
62
Issue
2
Year of publication
1993
Pages
251 - 262
Database
ISI
SICI code
0166-6851(1993)62:2<251:PNLPOL>2.0.ZU;2-3
Abstract
The proton nuclear magnetic resonance (NMR) lipid profile of Leishmani a donovani was obtained in the one-dimensional and two-dimensional mod es. Partial assignments of lipid classes and individual lipids were ob tained purely from the proton NMR spectrum of the mixture. A more comp lete assignment and quantitative analysis was achieved by prior separa tion of the lipids by high pressure liquid chromatography (HPLC) follo wed by proton NMR analysis of the fractions. This work showed that pro ton NMR spectroscopy could facilitate lipid analysis and classificatio n of various parasitic protozoa and serve as a basis for rapid studies of comparative lipid metabolism in parasites.