S. Meinecketillmann et al., SUPEROVULATION IN MERINO EWES WITH A SING LE FSH-P APPLICATION VS MULTIPLE INJECTIONS, Reproduction in domestic animals, 28(8), 1993, pp. 433-440
79 estrous synchronized sheep (Merinoland) were superovulated with FSH
-P (total dosis 25 mg or 17 mg). Group 1 got multiple FSH-P injections
i.m. in 12 h intervals (group 1/25 mg and group 1/17 mg), while in gr
oups 2 and 3 the total dosis was given subcutaneously 36 h before gest
agen withdrawal (group 2/25 mg and group 2/35 mg) or at the end of syn
chronisation (group 3/35 mg and group 3/17 mg). After intrauterine ins
emination embryo collection was performed on day 7-11 (day of estrous
= d0). In the different stimulation groups the number of ovulations wa
s 11,8 +/- 4,6; 9,2 +/- 5,5; 8,4 +/- 6,4; 7,2 +/- 7,2; 8,8 +/- 6,5 and
4,2 +/- 2,9, respectively. The reaction between group 3/17 mg and gro
ups 1/25 mg (p < 0,001), 1/17 mg (p < 0,01), 2/25 mg (p < 0,05) and 3/
25 mg (p < 0,05) differed significantly. The number of ovulations as w
ell as the number of number of embryos and oocytes recovered in the di
fferent experimental groups indicate that a single FSH application, es
pecially given in a higher dosis and at the end of estrus synchronisat
ion is a reasonable alternative for the superovulation regimen used no
rmally in sheep.