CAPILLARY GAS-CHROMATOGRAPHY COMBINED WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR THE STRUCTURAL-ANALYSIS OF OLIVE OIL TRIACYLGLYCEROLS

The triacylglycerol composition of olive oil samples has been determin ed by stereospecific analysis after partial hydrolysis with ethyl magn esium bromide, derivatization, preparative chiral HPLC, transesterific ation, and GC quantitation of fatty acid methyl esters. The data obtai ned for position sn-2 were compared with those from capillary GC analy sis of monoacyl sn-2-glycerols after enzymatic lipolysis of triacylgly cerols. The determination of triacylglycerols collected by silver ion HPLC and quantified (as fatty acid methyl esters) by GC, together with direct GC analysis on a polar column, have then furnished a comprehen sive picture of the triacylglycerol content of olive oil.