PREPARATION OF POROUS SPHERICAL POLY(GAMMA-METHYL L-GLUTAMATE) PARTICLES CONTAINING BETA-STRUCTURE

Porous poly(gamma-methyl L-glutamate) spherical particles underwent tr ansition alpha-helix-to-beta-structure in the main chain as a result o f heat treatment in water or methanol. The particles obtained maintain ed their sphericity and characteristic porosity after the heat treatme nt, while the transition increased insolubility against organic solven ts. The amount of beta-structure depended on the temperature. The tran sition in water included hydrolysis of the residual carbomethoxy group s. Adsorption capacity of the particles for cytochrome C depended rema rkably on the content of beta-structure as well as the amount of carbo xyl groups produced.