DIRECT ENANTIOSELECTIVE SEPARATION OF BEVANTOLOL BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON NORMAL AND REVERSE CELLULOSE CHIRAL STATIONARY PHASES

A direct, isocratic and simple chromatographic method is described for the enantiomeric separation Of bevantolol (BEV) using normal and reve rse cellulose tris (3,5-dimethylphenylcarbamate) chiral stationary pha ses (CSPs) known as Chiralcel OD and Chiralcel OD-R, respectively. The effect of various alcohols present in the mobile phase on the separat ion factor (alpha) and resolution factor (R(s)) has been studied. The method has been used to determine and identify the BEV enantiomers in human urine after oral administration of racemic BEV. The chiral recog nition mechanism(s) between the analyte and these chiral stationary ph ases is proposed.