RAPID ASSAY OF IOPANOIC ACID IN DOG PLASMA USING A HISEP COLUMN

Authors
ANDEEJANI AMI HUGHES H FEUCHUK DM ABOULENEIN HY
Citation
Ami. Andeejani et al., RAPID ASSAY OF IOPANOIC ACID IN DOG PLASMA USING A HISEP COLUMN, BMC. Biomedical chromatography, 8(1), 1994, pp. 26-28
Citations number
5
Categorie Soggetti
Chemistry Analytical","Pharmacology & Pharmacy",Biology
Journal title
BMC. Biomedical chromatography → ACNP
ISSN journal
02693879
Volume
8
Issue
1
Year of publication
1994
Pages
26 - 28
Database
ISI
SICI code
0269-3879(1994)8:1<26:RAOIAI>2.0.ZU;2-L
Abstract
A rapid method for the determination of iopanoic acid (IOP) in dog pla sma utilizing a Hisep column was developed. A mobile phase of 12% meth anol, 88% 0.05 M phosphate buffer pH 3.4 yielded a k' of 8.5 with no i nterference from proteins present in plasma. Recoveries of IOP from sp iked plasma ranged from 97% to 103% at 270 mumol/L and 1.75 mmol/L res pectively. Replication was +/-2.8% at 1.75 mmol/L and +/-6% at 21 mumo l/L. A method utilizing 2,4,6-triiodobenzoic acid as internal standard was also developed for comparison.