U. Neuenschwander et al., CYTOKININ INDUCES THE DEVELOPMENTALLY RESTRICTED SYNTHESIS OF AN EXTRACELLULAR PROTEIN IN PHYSCOMITRELLA-PATENS, Plant journal, 5(1), 1994, pp. 21-31
Early development of the moss Physcomitrella patens follows a simple c
ourse leading to the formation of a filamentous protonema containing o
nly two cell-types, chloronema and caulonema. The addition of the horm
one cytokinin leads to the induction of multicellular buds from such p
rotonema. The spectrum of extracellular proteins (ECPs) synthesized by
P. patens has been investigated at defined stages of development and
under defined hormone treatments. It is found that in contrast to the
limited changes in intracellular protein synthesis detectable, in the
extracellular environment major and specific changes in the patterns o
f proteins synthesized occur. For example, the presence of caulonema c
ells is characterized by the synthesis of a 25 kDa ECP whereas early c
hloronema differentiation is distinguished by the presence of a 38 kDa
ECP. The analysis of the pattern of ECPs synthesized by developmental
mutants altered in bud formation, and in response to cytokinin in tun
icamycin treated protonema (in which bud induction is blocked) indicat
e that the synthesis of a 14 kDa ECP is specifically induced by cytoki
nin. This protein represents a novel cytokinin-induced ECP. These data
show that the differentiation of particular cell types in plants is a
ssociated with the synthesis of particular ECPs, and suggest that horm
ones which induce specific morphogenic events may do so via the synthe
sis of specific ECPs.