RECOMBINATION OF LAC(-)MAL(PLUS) CELLS WITH LAC(PLUS) MAL-VARIANTS ARISEN SPONTANEOUSLY FROM DIFFERENT CANDIDA-ALBICANS STRAINS

Authors
SOUZA EMBE CURY AE FUNGARO MHP CAMARGO ZP DEAZEVEDO JL
Citation
Embe. Souza et al., RECOMBINATION OF LAC(-)MAL(PLUS) CELLS WITH LAC(PLUS) MAL-VARIANTS ARISEN SPONTANEOUSLY FROM DIFFERENT CANDIDA-ALBICANS STRAINS, Revista de Microbiologia, 24(3), 1993, pp. 156-160
Citations number
NO
Categorie Soggetti
Microbiology
Journal title
Revista de Microbiologia → ACNP
ISSN journal
00013714
Volume
24
Issue
3
Year of publication
1993
Pages
156 - 160
Database
ISI
SICI code
0001-3714(1993)24:3<156:ROLCWL>2.0.ZU;2-3
Abstract
Candida albicans strains, maintained in usual culture media, formed co lonies that, in auxonogram tests, showed an assimilation for lactose, but not for maltose. Spheroplasts prepared from these variants and fro m normal lac- mal+ cells of different origins were fused by the polyet hylene glycol method. This procedure yelded only lac+ mal+ cells. Afte r reduction in ploidy, these cells originated 82% of lac- mal+ colonie s, 13.3% of lac- mal- and 4.4% of lac+ mal+. Ploidy of the fusion prod uct, occurrence of heterocaryose in this product, and the loss of chro mosomes in the course of the segregation are discussed in this paper.