USE OF MONOCLONAL-ANTIBODIES FOR IMMUNOHISTOCHEMICAL STUDY OF BOVINE LYMPH-NODES ON FROZEN-SECTIONS

Many monoclonal antibodies reactive with bovine leukocyte differentiat ion antigens are now available. Immunohistochemical staining on frozen sections using these monoclonal antibodies permits study of the funct ional morphology of bovine lymph nodes. Our study confirms usually acc epted notions (B and T dependent-zones) and supplies complementary dat a about the repartition of CD4 cells (particularly intrafollicular pos itive cells), gammadelta T cells, MHC II expression and dendritic leuk ocytes.