IN-VITRO MODULATION OF INTERLEUKIN-1-BETA SECRETION BY CULTURED RAT DOXORUBICIN-STIMULATED WHOLE GLOMERULI AND DISSOCIATED MESANGIAL GLOMERULAR CELLS

Doxorubicin-stimulated whole rat glomeruli and dissociated mesangial a nd resident glomerular macrophage cells produced the release of interl eukin (IL)-1 beta cytokine. This activity increased after the addition of lipopolysaccharide (LPS) or LPS plus indomethacin to the cultures. In the presence of WEB2086 [platelet-activating factor (PAF)-acether antagonist], this activity showed a drastic reduction, without modific ation after sodium furegrelate (throboxane synthetase inhibitor) was a dded to the cultures. Our results also demonstrate that this IL-1 beta activity is mainly produced by glomerular-resident macrophage cells. These findings support the important role by both IL-1 beta and PAF-ac ether mediator factors, at the cellular level, in the rat model of dox orubicin-induced nephrosis.