T. Bricio et al., IN-VITRO MODULATION OF INTERLEUKIN-1-BETA SECRETION BY CULTURED RAT DOXORUBICIN-STIMULATED WHOLE GLOMERULI AND DISSOCIATED MESANGIAL GLOMERULAR CELLS, Immunology, 81(1), 1994, pp. 53-57
Doxorubicin-stimulated whole rat glomeruli and dissociated mesangial a
nd resident glomerular macrophage cells produced the release of interl
eukin (IL)-1 beta cytokine. This activity increased after the addition
of lipopolysaccharide (LPS) or LPS plus indomethacin to the cultures.
In the presence of WEB2086 [platelet-activating factor (PAF)-acether
antagonist], this activity showed a drastic reduction, without modific
ation after sodium furegrelate (throboxane synthetase inhibitor) was a
dded to the cultures. Our results also demonstrate that this IL-1 beta
activity is mainly produced by glomerular-resident macrophage cells.
These findings support the important role by both IL-1 beta and PAF-ac
ether mediator factors, at the cellular level, in the rat model of dox
orubicin-induced nephrosis.