A. Korfel et al., MACROPHAGE INFLAMMATORY PROTEIN (MIP)-1-ALPHA STEM-CELL INHIBITOR (SCI) DOES NOT AFFECT CLONAL GROWTH OF HUMAN SOLID TUMOR-CELL LINES IN-VITRO, International journal of oncology, 4(2), 1994, pp. 353-357
MIP-1alpha is a member of a family of proinflammatory cytokines produc
ed by activated macrophages which has been shown to be a negative regu
lator of early hematopoietic stem cell progenitors. We report on resul
ts testing recombinant human (rh) MIP-1alpha on the clonal growth of d
ifferent human nonhematopoietic tumor cell lines in vitro. Cell lines
tested included the following histologies: 7 glioblastomas, 1 neurobla
stoma, 2 head and neck carcinomas, 4 lung carcinomas, 3 colorectal car
cinomas, 1 gastric carcinoma, 1 pancreatic carcinoma, 1 breast carcino
ma, 1 prostate carcinoma, 1 choriocarcinoma, 1 ovary carcinoma, 1 oste
osarcoma, and 3 melanomas. MIP-1alpha (0, 2, 20, 200 ng/ml) was tested
in human tumor cloning assays (HTCA) in agar-containing capillaries (
HTCAcap) and in mixtures of methylcellulose and agar (HTCAmix), repres
enting assay systems with different plating efficiencies (PE). Tumor c
ells were continuously exposed to the cytokine for the complete assay
period. Clonal growth of none of the cell lines was significantly and
reproducibly stimulated or inhibited by MIP-1alpha.