In the paper a study about the reactive extraction of Penicilliln V, f
or the water-butylacetate system, is reported. The reactive extraction
was carried out with Amberlit LA-2 in a glass column, at constant tem
perature, using a pulsatory mixer. The influence of pH-value, amine co
ncentration in organic phase, extraction time, Penicillin V concentrat
ion in aqueous phase, volumetric ratio aqueous phase : organic phase o
n the separation yield has been followed.