ENZYME-ENTRAPPING MEMBRANES FOR BIOSENSORS OBTAINED BY RADIATION-INDUCED POLYMERIZATION

A new method of physically immobilizing enzymes in poly(2-hydroxyethyl methacrylate) membranes was developed in order to obtain suitable bio sensors. It was possible to prepare an enzyme sensor based on an oxyge n Clark electrode and on glucose oxidase immobilized by low-temperatur e gamma radiation-induced polymerization. Temperature and pH effects o n the activity of immobilized enzyme are described and the response ch aracteristics of the resulting biosensor are summarized. The determina tion of glucose in standard solutions was carried out and a linear cal ibration curve, with an R(2) value of 0.9993, from the detection limit 5 x 10(-5) M to 1.2 x 10(-3) M was obtained. The biosensor was employ ed to analysis of control sera and the results were compared to those obtained by enzymatic-spectrophotometric detection.