L. Bonneau et al., SOMATIC EMBRYOGENESIS AND PLANT-REGENERATION IN A WOODY SPECIES - THEEUROPEAN SPINDLE TREE (EUONYMUS-EUROPAEUS L), Plant cell reports, 13(3-4), 1994, pp. 135-138
Somatic embryogenesis and subsequent plant regeneration of Euonymus eu
ropaeus L (European Spindle Tree) were obtained from square pieces of
mature zygotic embryos with an intervening callus phase. Callus and so
matic embryos were induced using a Murashige and Skoog's semi-solid ba
sal medium supplemented with several combinations of auxins and cytoki
nins. The greatest number of somatic embryos was obtained with a conti
nuous exposure to 22.8 muM indoleacetic acid and 0.046 muM kinetin. Th
e frequency of somatic embryogenesis from zygotic embryos depends on t
he cold conservation time of seeds. The embryos frequently germinated
on the same medium. Further development of somatic embryos into plantl
ets was achieved on a medium devoid of growth regulators.