IN-VITRO MULTIPLICATION AND FIELD ESTABLISHMENT OF ADHATODA-BEDDOMEI CB CLARKE, A RARE MEDICINAL PLANT

Authors
Citation
Cg. Sudha et S. Seeni, IN-VITRO MULTIPLICATION AND FIELD ESTABLISHMENT OF ADHATODA-BEDDOMEI CB CLARKE, A RARE MEDICINAL PLANT, Plant cell reports, 13(3-4), 1994, pp. 203-207
Citations number
14
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
07217714
Volume
13
Issue
3-4
Year of publication
1994
Pages
203 - 207
Database
ISI
SICI code
0721-7714(1994)13:3-4<203:IMAFEO>2.0.ZU;2-B
Abstract
Clonal propagation of Adhatoda beddomei C.B. Clarke (Acanthaceae), a r are medicinal shrub, was achieved through callus-free axillary meriste m proliferation from stem node explants of field-grown plants cultured in SH medium. Shoot multiplication was a function of cytokinin activi ty but sustained growth of the shoots was dependent on the synergistic effect with the auxin, IAA. An optimum number of 5-10 shoots per expl ant were obtained in 6 weeks using 3.0 mg.l-1 BAP, 0.5 mg.l-1 2-ip and 1.0 mg.l-1 IAA. Upon subculture, vertical halves of the precultured n ode with the differentiated shoots yielded a larger aggregate number o f shoots (23-27) than the uncut precultured node left intact (15-17). Shoot multiplication was rapid and consistent over prolonged periods w hen the hormonal concentrations were reduced to 1.0 mg.l-1 BAP and 0.2 mg.l-1 IAA during subculture, and reculture of the nodal explants der ived from shoot cultures. Rooting of 3-5 cm shoots thus obtained was g reatly accelerated in stationary liquid medium containing 0.2 mg.l-1 I BA or IAA. Hardening ok the rooted plantlets in the humidity chamber w as essential for high frequency (95%) survival. Micropropagated plants established in the field flowered after fifteen months and were free from apparent defects in cytological, growth and flowering characteris tics.