COMPONENTS PREPARED FROM BLOOD COLLECTED INTO DEXTROSE-FREE ANTICOAGULANT - IN-VITRO PROPERTIES OF PLATELETS SUSPENDED IN PLASMA AND IN DEXTROSE-FREE SYNTHETIC MEDIUM
A. Farrugia et al., COMPONENTS PREPARED FROM BLOOD COLLECTED INTO DEXTROSE-FREE ANTICOAGULANT - IN-VITRO PROPERTIES OF PLATELETS SUSPENDED IN PLASMA AND IN DEXTROSE-FREE SYNTHETIC MEDIUM, Transfusion science, 14(4), 1993, pp. 409-416
Blood donations were collected into dextrose-free citrate (C) anticoag
ulant, so as to result in citrate levels equal to those found in stand
ard anticoagulant (CPD). Platelet concentrates (PCs) prepared from the
se donations were suspended in plasma or in a dextrose-free synthetic
medium. In paired studies comparing storage in different plastics, pH
was adequately maintained when C platelets were stored in Fenwal PL124
0 or PL146, while in C platelets supplemented with dextrose pH levels
were only maintained in PL1240. In addition, platelet counts in C plat
elets stored in PL1240 showed no relationship with the pH at the end o
f storage (R=0.13), while a strong correlation was found for CPD plate
lets (R=-0.76). In vitro tests of platelet quality showed that C plate
lets suspended in plasma or in the synthetic medium showed similar or
superior properties to C platelets suspended in plasma supplemented wi
th dextrose. These findings suggest that dextrose from plasma carry ov
er is not responsible for maintaining previously reported improvements
in the quality of platelets in a dextrose-free synthetic medium. It i
s suggested that exclusion of dextrose from the platelet's storage env
ironment allows platelet pH and in vitro quality to be maintained with
out the constraints of platelet count or storage plastic.