Am. Dopico et al., MEMBRANE STRETCH DIRECTLY ACTIVATES LARGE-CONDUCTANCE CA2-ACTIVATED K+ CHANNEIS IN MESENTERIC-ARTERY SMOOTH-MUSCLE CELLS(), American journal of hypertension, 7(1), 1994, pp. 82-89
Large-conductance, Ca2+-activated K+ channels were identified in singl
e smooth muscle cells freshly isolated from rabbit superior mesenteric
artery. They typically showed a reversal potential close to 0 mV in e
xcised, inside-out patches in symmetric 130 mmol/L [K+] with a unitary
conductance of 260 pS, and increased activity at more positive potent
ials and/or when [Ca2+] was raised at the cytosolic surface of the mem
brane. Both in cell-attached and in excised, inside-out configurations
, stretching the membrane patch by applying suction to the back of the
patch pipette increased the activity of these channels without changi
ng either the unitary conductance or the voltage sensitivity of the ch
annel. Stretch activation was repeatedly seen in inside-out patches wh
en both surfaces were bathed with a 0 Ca2+ solution containing 2 or 5
mmol/L EGTA to chelate trace amounts of Ca2+, making it highly improba
ble that stretch activation could be secondary to a stretch-induced fl
ux of Ca2+. Consequently, stretch activation of large-conductance, Ca2
+-activated K+ channels in mesenteric artery smooth muscle cells seems
to be due to a direct effect of stretch on the channel itself or on s
ome closely associated, membrane-bound entity.