MEMBRANE STRETCH DIRECTLY ACTIVATES LARGE-CONDUCTANCE CA2-ACTIVATED K+ CHANNEIS IN MESENTERIC-ARTERY SMOOTH-MUSCLE CELLS()

Citation
Am. Dopico et al., MEMBRANE STRETCH DIRECTLY ACTIVATES LARGE-CONDUCTANCE CA2-ACTIVATED K+ CHANNEIS IN MESENTERIC-ARTERY SMOOTH-MUSCLE CELLS(), American journal of hypertension, 7(1), 1994, pp. 82-89
Citations number
NO
Categorie Soggetti
Cardiac & Cardiovascular System
ISSN journal
08957061
Volume
7
Issue
1
Year of publication
1994
Pages
82 - 89
Database
ISI
SICI code
0895-7061(1994)7:1<82:MSDALC>2.0.ZU;2-1
Abstract
Large-conductance, Ca2+-activated K+ channels were identified in singl e smooth muscle cells freshly isolated from rabbit superior mesenteric artery. They typically showed a reversal potential close to 0 mV in e xcised, inside-out patches in symmetric 130 mmol/L [K+] with a unitary conductance of 260 pS, and increased activity at more positive potent ials and/or when [Ca2+] was raised at the cytosolic surface of the mem brane. Both in cell-attached and in excised, inside-out configurations , stretching the membrane patch by applying suction to the back of the patch pipette increased the activity of these channels without changi ng either the unitary conductance or the voltage sensitivity of the ch annel. Stretch activation was repeatedly seen in inside-out patches wh en both surfaces were bathed with a 0 Ca2+ solution containing 2 or 5 mmol/L EGTA to chelate trace amounts of Ca2+, making it highly improba ble that stretch activation could be secondary to a stretch-induced fl ux of Ca2+. Consequently, stretch activation of large-conductance, Ca2 +-activated K+ channels in mesenteric artery smooth muscle cells seems to be due to a direct effect of stretch on the channel itself or on s ome closely associated, membrane-bound entity.