AMINOGUANIDINE REDUCES REGIONAL ALBUMIN CLEARANCE BUT NOT URINARY ALBUMIN EXCRETION IN STREPTOZOTOCIN-DIABETIC RATS

Advanced glycation end-product-formation is thought to play a role in the development of diabetic angiopathy. By altering the structure of d ifferent extracellular matrix components advanced glycation endproduct s might affect vascular and glomerular permeability. In this study we investigated the effect of treatment with an inhibitor of advanced gly cation end-product-formation, aminoguanidine, on vascular permeability and the development of albuminuria in streptozotocin-induced diabetic rats. Male Wistar Rp rats were randomized into a control group, a dia betic group, and an aminoguanidine-treated diabetic group. After 8 wee ks, 24-h urine collections were taken and rats were implanted with an arterial and a venous catheter. Mean arterial blood pressure was deter mined by intra-arterial measurement. Regional albumin clearances were assessed in the eye, ileum, lung, skeletal muscle and skin using an is otope technique. Mean arterial pressure in the diabetic group was sign ificantly lower in the control and aminoguanidine-treated groups (p < 0.02). Regional albumin clearances were significantly increased in all tissues of diabetic rats compared to control rats (p < 0.05). Aminogu anidine treatment of diabetic rats resulted in a significant decrease of regional albumin clearance in all tissues except the lung (p < 0.05 , lung p = 0.07). The development of albuminuria in diabetic rats howe ver, was not affected by aminoguanidine.