ONLINE IMMUNOANALYSIS FOR BIOPROCESS CONTROL

Immunoanalytical techniques such as ELISA are often used for the detec tion of proteins produced in cultivation processes. Owing to the diffi culty of automating of the time-consuming traditional ELISA, there is an intense demand for a suitable on-line monitoring method. Combining well-known immunoassays with the FIA technique, we present the heterog eneous and the turbidimetric immuno-FIA methods. The following protein s were investigated with these FIA methods: thermostable pullulanase, IgG, antithrombin III, and recombinant tissue-type plasminogen activat or. In the cases of pullulanase and monoclonal mouse IgG, the turbidim etric immuno-FIA was used for on-line analysis of the cultivation proc ess. Results are presented here to demonstrate the effectiveness and a pplication of these immunoanalyses.