PROTEIN-KINASE-C SUBSPECIES IN HIPPOCAMPUS AND STRIATUM OF RESERPINIZED RAT-BRAIN

Protein kinase C (PKC) (alpha) and (beta) immunoreactivities were dete rmined in the cytosolic and particulate fractions of the hippocampus a nd striatum after single and repetitive, intraperitoneal administratio n of reserpine. Significant decrease of PKC(alpha) immunoreactivities was observed in the particulate fractions of both striatum and hippoca mpus. Compared with the alteration of PKC(alpha), the decreased immuno reactivity of PKC(beta) was only observed in the particulate fraction of the hippocampus, associated with the increase in the cytosolic frac tion. These findings suggest a novel mechanism regulating the distribu tion of PKC. The topographical selectivity in the present study may in dicate the importance of the alteration of PKC(beta) in the pathophysi ological mechanism in Alzheimer's disease.