EPITOPE COMPLEMENTARITY AND IDIOTYPIC INTERACTIONS - A STUDY OF IDIOTYPIC-LIKE INTERACTIONS BETWEEN ANTI-CYTIDINE AND ANTI-GUANOSINE A J-MOUSE MONOCLONAL-ANTIBODIES .1. CHARACTERIZATION OF THESE INTERACTIONS/
Mh. Cottet et al., EPITOPE COMPLEMENTARITY AND IDIOTYPIC INTERACTIONS - A STUDY OF IDIOTYPIC-LIKE INTERACTIONS BETWEEN ANTI-CYTIDINE AND ANTI-GUANOSINE A J-MOUSE MONOCLONAL-ANTIBODIES .1. CHARACTERIZATION OF THESE INTERACTIONS/, Molecular immunology, 31(1), 1994, pp. 65-74
Idiotypic-like interactions between mAbs directed against cytidine (Cy
d) or guanosine (Guo) nucleosides were characterized. These mAbs, Cyd-
1 (IgG2b, kappa), Guo-1 (IgG1, kappa) and Guo-2 (IgG1, kappa) were der
ived from splenocytes of A/J mice immunized with Cyd-KLH or Guo-KLH an
d recognized the nucleoside base moieties involved in hydrogen bonding
. The interactions between Guo-1 or Guo-2 and Cyd-1 involved cross-rea
ctive or distinct-but-neighboring paratope-associated idiotopes. These
interactions were characterized by K(D) values of 4.6 x 10(-6) and 1.
8 x 10(-6) M, respectively. The three anti-nucleoside mAbs exhibited A
b2beta properties and manifested epibody (Ab2epsilon) activity towards
ssDNA. We compared these idiotypic-like reactivities with the anti-id
iotypic activity of an intentionally induced IgG1, kappa anti-idiotype
mAb prepared with splenocytes from A/J mice immunized with Cyd-1. Thi
s Ab2 antibody which bound to Cyd-1 with a K(D) of 1.1 x 10(-9) M, man
ifested an Ab2gamma activity, i.e. it recognized a paratope-associated
idiotope on Cyd-1 without exhibiting Ab2beta properties. In addition,
the anti-(Cyd-1) completely inhibited (Cyd-1)-(Guo-1) and (Cyd-1)-(Gu
o-2) interactions.