INDUCTION OF THE LYTIC VIRAL CYCLE IN EPSTEIN-BARR-VIRUS CARRYING BURKITT-LYMPHOMA LINES IS ACCOMPANIED BY INCREASED EXPRESSION OF MAJOR HISTOCOMPATIBILITY COMPLEX-MOLECULES

Six Epstein Barr virus (EBV) genome-carrying Burkitt lymphoma (BL) cul tures (P3HR-1, Raji, Akata, Daudi, Rael and Jijoye) were induced to en ter the lytic cycle. Phorbol esther (TPA), n-butyrate, 5-azacytidine ( 5AzaC) or anti-IgG were used according to their known inducing capacit y on these cell lines. Concomitantly with the appearance of the viral early antigens (EA) in a proportion of cells, the expression of major histocompatibility complex (MHC) class II antigens increased in the cu ltures. On P3HR-1 and Raji cells class I expression also increased. Th e enhancement of MHC expression correlated with the efficiency of indu ction and required only an early event of the viral lytic cycle. Treat ment of 3 EBV-negative lymphoma lines (BJAB, Ramos and BL41) with TPA plus n-butyrate or 5AzaC did not influence MHC expression. Moreover, B L lines which carry the EBV genome after having been infected in vitro and which cannot be induced for the viral lytic cycle did not change MHC expression after treatment with theinducing agents. In mixed cultu res the allo-stimulatory capacity of induced cells with elevated MHC e xpression was stronger compared to the untreated ones.