Kw. Linz et K. Kohler, ISOLATION OF PROTOPLASTS FROM THE COCCAL GREEN-ALGA EREMOSPHAERA-VIRIDIS DEBARY FOR PATCH-CLAMP MEASUREMENTS, Botanica acta, 106(6), 1993, pp. 469-472
A method for enzymatic isolation of protoplasts from the unicellular g
reen alga Eremosphaera viridis for patch-clamp measurements is describ
ed. Viable protoplasts with ''patch-clean'' plasma membranes could onl
y be isolated when combining high enzyme concentrations and long incub
ation times. In whole-cell recordings the protoplasts exhibited electr
ical properties similar to those measured in intact cells. Taken toget
her with the protoplasts' ability for rapid deplasmolysis after transf
er into hypotonic solution, this indicates the viability of the isolat
ed protoplasts.