SPECTROPHOTOMETRIC AND CHROMATOGRAPHIC (HPLC) ANALYSIS OF HYDRALAZINE, DIHYDRALAZINE AND HYDRAZINE AFTER DERIVATIZATION WITH 2-NITROCINNAMALDEHYDE

Authors
DIPIETRA AM ROVERI P GOTTI R CAVRINI V
Citation
Am. Dipietra et al., SPECTROPHOTOMETRIC AND CHROMATOGRAPHIC (HPLC) ANALYSIS OF HYDRALAZINE, DIHYDRALAZINE AND HYDRAZINE AFTER DERIVATIZATION WITH 2-NITROCINNAMALDEHYDE, Il Farmaco, 48(11), 1993, pp. 1555-1567
Citations number
25
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
Il Farmaco → ACNP
ISSN journal
0014827X
Volume
48
Issue
11
Year of publication
1993
Pages
1555 - 1567
Database
ISI
SICI code
0014-827X(1993)48:11<1555:SAC(AO>2.0.ZU;2-J
Abstract
A simple colorimetric method, based on the reaction with 2-nitrocinnam aldehyde, has been developed for the determination of hydralazine (lam bda = 390 nm) and dihydralazine (lambda = 395 nm) in their dosage form s. The method proved to be stability-indicating and provided results c omparable to those obtained by a reference HPLC (cyano column) method. Prechromatographic derivatization with 2-nitrocinnamaldehyde, in comb ination with a preliminary solid-phase extraction (C-18 sorbent), enab led sensitive and selective HPLC determinations of hydrazine in hydral azine to be accomplished.