GENETIC-DEFECT OF THE SODIUM PUMP-DEFECTIVE MUTANT NAP1 FROM THE MARINE VIBRIO-ALGINOLYTICUS

Authors
HAYASHI M FUJII J UNEMOTO T
Citation
M. Hayashi et al., GENETIC-DEFECT OF THE SODIUM PUMP-DEFECTIVE MUTANT NAP1 FROM THE MARINE VIBRIO-ALGINOLYTICUS, Biochemistry and molecular biology international, 41(1), 1997, pp. 41-47
Citations number
16
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
41
Issue
1
Year of publication
1997
Pages
41 - 47
Database
ISI
SICI code
1039-9712(1997)41:1<41:GOTSPM>2.0.ZU;2-E
Abstract
The marine bacterium, Vibrio alginolyticus, has a respiratory chain-li nked Na+-translocating NADH-quinone reductase (NQR). Among several mut ant cells defective in Na+ pump activity, Nap1 was a very stable mutan t and a spontaneous revertant could not be isolated from Nap1. Using g enetic information from the recently sequenced nqr operon, the genetic defects in Nap1 were examined, and the sodium pump-defective mutant N ap1 was found to be caused by the insertion of a 1.2 kbp DNA fragment into the C-terminal region of nqr6 gene.