IDENTIFICATION OF A ZN2-SENSITIVE COMPONENT OF EHRLICH CELL PLASMA-MEMBRANE REDOX SYSTEM BY CHAPS-AGAROSE-POLYACRYLAMIDE ELECTROPHORESIS AND IN-SITU STAINING OF ACTIVITY()

Authors
RODRIGUEZCASO L RODRIGUEZAGUDO D DELCASTILLOOLIVARES A MARQUEZ J DECASTRO IN MEDINA MA
Citation
L. Rodriguezcaso et al., IDENTIFICATION OF A ZN2-SENSITIVE COMPONENT OF EHRLICH CELL PLASMA-MEMBRANE REDOX SYSTEM BY CHAPS-AGAROSE-POLYACRYLAMIDE ELECTROPHORESIS AND IN-SITU STAINING OF ACTIVITY(), Biochemistry and molecular biology international, 41(1), 1997, pp. 75-81
Citations number
27
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
41
Issue
1
Year of publication
1997
Pages
75 - 81
Database
ISI
SICI code
1039-9712(1997)41:1<75:IOAZCO>2.0.ZU;2-4
Abstract
A procedure based on CHAPS-agarose-polyacrylamide electrophoresis and in situ staining of activity was used to detect a Zn2+-sensitive compo nent of Ehrlich cell plasma membrane redox system. The procedure is so powerful that it allows to use elude plasma membrane fractions and ca n be easily adapted for use in an electrophoretic approach to the puri fication of this protein.