T. Solouki et al., MASS MEASUREMENT ACCURACY OF MATRIX-ASSISTED LASER-DESORBED BIOMOLECULES - A FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETRY STUDY, Rapid communications in mass spectrometry, 8(1), 1994, pp. 26-31
The use of binary matrices and internal calibrants to improve the mass
measurement accuracy in matrix-assisted laser desorption ionization (
MALDI) with Fourier-transform ion cyclotron resonance (FTICR) mass spe
ctrometry is described. Binary matrices enhance the analyte ion yield
and enable a complete MALDI-FTICR mass spectrum to be obtained from a
single laser shot. The advantage of single-laser-shot data acquisition
is that it eliminates line-broadening due to shot-to-shot frequency v
ariations. It is shown that unresolved product ions, mainly due to los
s of H2O and/or NH3, shift the centroid of an unresolved multi-compone
nt peak. A mass measurement accuracy of 12 ppm was obtained for the bo
vine insulin [M + H]+ ion using melittin as an internal calibrant.