MASS MEASUREMENT ACCURACY OF MATRIX-ASSISTED LASER-DESORBED BIOMOLECULES - A FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETRY STUDY

Citation
T. Solouki et al., MASS MEASUREMENT ACCURACY OF MATRIX-ASSISTED LASER-DESORBED BIOMOLECULES - A FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETRY STUDY, Rapid communications in mass spectrometry, 8(1), 1994, pp. 26-31
Citations number
38
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
ISSN journal
09514198
Volume
8
Issue
1
Year of publication
1994
Pages
26 - 31
Database
ISI
SICI code
0951-4198(1994)8:1<26:MMAOML>2.0.ZU;2-5
Abstract
The use of binary matrices and internal calibrants to improve the mass measurement accuracy in matrix-assisted laser desorption ionization ( MALDI) with Fourier-transform ion cyclotron resonance (FTICR) mass spe ctrometry is described. Binary matrices enhance the analyte ion yield and enable a complete MALDI-FTICR mass spectrum to be obtained from a single laser shot. The advantage of single-laser-shot data acquisition is that it eliminates line-broadening due to shot-to-shot frequency v ariations. It is shown that unresolved product ions, mainly due to los s of H2O and/or NH3, shift the centroid of an unresolved multi-compone nt peak. A mass measurement accuracy of 12 ppm was obtained for the bo vine insulin [M + H]+ ion using melittin as an internal calibrant.