IMMUNOREACTIVITIES OF P11 AND CALCYCLIN IN BAKERS-YEAST, WHEAT-GERM AND LOBSTER

After effectively eliminating the nonspecific cross-immunoreactivity w ith the affinity columns of anti-IgG agarose and IgG agarose, the pote nt immunoreactivities of p11 and calcyclin in wheat germ, lobster tail muscle, and three strains of baker's yeast were analyzed by Western b lotting using mouse anti-pll and rabbit anti-calcyclin. The occurrence of multiple bands may be due to either autolyses and/or the interacti ons between the pll (or calcyclin) and other endogenous biological mol ecules. The results suggest not only a ubiquitous distribution and a u niversal Ca2+-mediating regulatory role of pll and calcyclin in eukary otes, but also an evolutionary conservation of these (S-100)-related p roteins.