GENOMIC CLONING AND PARTIAL CHARACTERIZATION OF HUMAN CHYMOTRYPSINOGEN GENE

Chymotrypsinogen is a principal precursor of pancreatic proteolytic en zymes. We previously isolated a cDNA clone for human prechymotrypsinog en from a human pancreatic cDNA library. In the present study, we used this cDNA sequences to isolate genomic DNA clones. Three overlapping cosmid clones spanning approximately 65-kb genomic sequences were isol ated from a human cosmid library. The genomic DNA clones were characte rized by restriction enzyme mapping and by hybridizing them to subfrag ments of the cDNA. The sequence tagged sites for human chymotrypsinoge n gene were created by designing two oligonucleotides. Furthermore, th e isolated genomic clones were confirmed to be localized on chromosome 16q23 by fluorescence in situ hybridization and G-banding analysis.