PRODUCTION OF A SPECIFIC MONOCLONAL-ANTIBODY TO TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE (TDT) AND THE EXTENSIVE STUDIES OF TDT IN PATIENTS WITH HEMATOLOGICAL MALIGNANCIES

Authors
SASAKI R YUASA Y MASUYAMA A TAKAKU F BOLLUM FJ
Citation
R. Sasaki et al., PRODUCTION OF A SPECIFIC MONOCLONAL-ANTIBODY TO TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE (TDT) AND THE EXTENSIVE STUDIES OF TDT IN PATIENTS WITH HEMATOLOGICAL MALIGNANCIES, Haematologia, 25(4), 1993, pp. 223-235
Citations number
NO
Categorie Soggetti
Hematology
Journal title
Haematologia → ACNP
ISSN journal
00176559
Volume
25
Issue
4
Year of publication
1993
Pages
223 - 235
Database
ISI
SICI code
0017-6559(1993)25:4<223:POASMT>2.0.ZU;2-V
Abstract
Terminal deoxynucleotidyl transferase (TdT) was purified from calf thy mus and a monoclonal antibody-producing clone was produced by the fusi on of mouse myeloma cells and spleen cells of mice immunized with the purified enzyme. The antigens recognized by an immunoadsorbent column revealed TdT, whose molecular weight was 62 kDa. The extensive study o f TdT in 196 patients with hematological disorders was done. The resul ts from immunofluorescent analysis corresponded well to the results of biochemical assays of this enzyme. This monoclonal antibody will prov ide a useful means for the survey of leukemia and lymphoma patients.