N. Kleinbergerdoron et Bi. Kanner, IDENTIFICATION OF TRYPTOPHAN RESIDUES CRITICAL FOR THE FUNCTION AND TARGETING OF THE GAMMA-AMINOBUTYRIC-ACID TRANSPORTER (SUBTYPE-A), The Journal of biological chemistry, 269(4), 1994, pp. 3063-3067
The gamma-aminobutyric acid transporter is localized in nerve terminal
s. It catalyzes coupled electrogenic translocation of the neurotransmi
tter with two or three sodium ions and one chloride ion. The transport
er contains 599 amino acids and 12 putative membrane spanning alpha-he
lices. It is the first described member of a neurotransmitter transpor
ter superfamily. Using site-directed mutagenesis we have investigated
the role of all 10 tryptophan residues predicted to reside in these he
lices. All 10 have been changed to serine as well as to leucine residu
es. Expression of mutant cDNAs in which the tryptophans, located in po
sitions 68, 222, and 230, are replaced by either of these two amino ac
ids reveals that they are severely impaired in gamma-aminobutyric acid
transport. Mutants in which a phenylalanine or a tyrosine residue is
introduced, at either position 68 or 230, are active. On the other han
d, at the 222 position replacement of the tryptophan by the aromatic a
mino acids results in inactive transport. After prelabeling of the pro
teins with [S-35]methionine, immunoprecipitation of mutant transporter
s indicates that their expression levels are similar to those of the w
ild type. Reconstitution experiments, aimed to reveal the activity of
transporter molecules not apparent in the plasma membrane, indicate th
at the lack of activity of the W230S transporter in intact cells is by
and large due to its inefficient targeting to the plasma membrane. Tr
yptophan residues 68 and 222 appear to be required for the intrinsic a
ctivity of the transporter. Based on several observations, including o
ne that tryptophan residue 222 is conserved in all amino acid transpor
ter members of the superfamily, but not in those transporting biogenic
amines, we hypothesize that the pi electrons of this tryptophan could
be involved in the binding of the amino group of these neurotransmitt
ers.