Qh. Xu et al., CLONING AND IDENTIFICATION OF TESTIS-SPECIFIC TRANSCRIPTION ELONGATION FACTOR-S-II, The Journal of biological chemistry, 269(4), 1994, pp. 3100-3103
A new S-II cDNA clone was isolated from a rat testis library. This cDN
A contained an open reading frame encoding 299 amino acid residues. Th
e deduced amino- and carboxyl-terminal regions were very similar with
those of Ehrlich cell S-II, which we reported previously, but the sequ
ence of the intervening 46 amino acid residues was unique. This new S-
II was expressed in the testis but not in the other rat tissues examin
ed, suggesting that it was a testis-specific S-II. Recombinant testis-
specific S-II produced in Escherichia coli was shown to stimulate RNA
polymerase II.