H. Ensinger et al., GLUCOSE AND UREA PRODUCTION AND LEUCINE, KETOISOCAPROATE AND ALANINE FLUXES AT SUPRAPHYSIOLOGICAL PLASMA ADRENALINE CONCENTRATIONS IN VOLUNTEERS, Intensive care medicine, 20(2), 1994, pp. 113-118
Objective. To determine the magnitude and time course of adrenergic ef
fects on metabolism in volunteers and possible implications for the us
e of sympathomimetics in the critically ill. Design: Descriptive labor
atory investigation. Subjects: 7 volunteers. Intervention: Primed cont
inuous infusions of stable isotope tracers ([N-15(2)]-urea, [6,6-D2]-g
lucose, [methyl-D3]-L-leucine, [N-15]-L-alanine) were used. After isot
opic steady state had been reached an infusion of adrenaline (0.1 mug/
kg/min) was administered (4 h). Isotopic enrichment was measured using
gas chromatography-mass spectrometry and the corresponding rates of a
ppearance were calculated. Measurements and main results: Glucose prod
uction increased from 14.1 +/- 1.2 to 21.5 +/- 2.0 mumol/kg/min (p < 0
.05) after 80 min of adrenergic stimulation and then decreased again t
o 17.9 +/- 1.2 mumol/kg/min after 240 min. Leucine and ketoisocaproate
(KIC) fluxes were 2.3 +/- 0.2 and 2.6 +/- 0.2 mumol/kg/min, respectiv
ely, at baseline and gradually decreased to 1.8 +/- 0.2 and 2.2 +/- 0.
1 mumol/kg/min, respectively, after 240 min of adrenaline infusion (bo
th p < 0.05). Alanine flux increased from 3.7 +/- 0.5 to 6.9 +/- 0.9 m
umol/kg/min (p < 0.05) after 80 min of adrenergic stimulation. Urea pr
oduction slightly decreased from 4.8 +/- 0.9 to 4.3 +/- 0.8 mumol/kg/m
in during adrenaline (p < 0.05). Conclusions. Adrenaline induced an in
crease in glucose production lasting for longer than 240 min. The decr
ease in leucine and KIC flux suggests a reduction in proteolysis, whic
h was supported by the decrease in urea production. The increase in al
anine flux is therefore most likely due to an increase in de-novo synt
hesis. The ammonia donor for alanine synthesis in peripheral tissues a
nd the target for ammonia after alanine deamination in the liver remai
n to be investigated. These results indicate that adrenaline infusion
most probably will not promote already enhanced proteolysis in critica
lly ill patients. Gluconeogenesis is an energy consuming process and a
n increase may deteriorate hepatic oxygen balance in patients.