M. Viluksela et al., DITHRANOL (ANTHRALIN) AND L0-BUTYRYL DITHRANOL (BUTANTRONE) DO NOT MORPHOLOGICALLY TRANSFORM CULTURED C3H 10T1 2 C18 MOUSE EMBRYO FIBROBLASTS/, Acta dermato-venereologica, 74(1), 1994, pp. 54-56
The ability of dithranol and 10-butyryl dithranol to induce morphologi
cal cell transformation was studied in cultured C3H 10T1/2 C18 fibrobl
asts. The cells were incubated with different concentrations of the te
st compounds for 48 h and cultured for 5 weeks thereafter. At the end
of the culture period the cultures were fixed, stained and examined fo
r the presence of transformed foci. Dithranol and 10-butyryl dithranol
did not increase the formation of transformed foci, while the positiv
e control compound, 7,12-dimethylbenz(a)anthracene (DMBA), induced a h
igh frequency of transformations significantly different from controls
. Thus the in vitro cell transformation model with uninitiated C3H 10T
1/2 C18 fibroblasts is not able to detect the weak tumorigenic action
of dithranol and 10-butyryl dithranol which has been observed in mouse
skin.