Ae. Fine et al., EFFECTS OF TIME, FROM COLLECTION TO PROCESSING, ON THE RECOVERY OF HAEMONCHUS-CONTORTUS 3-STAGE LARVAE FROM HERBAGE, Veterinary parasitology, 51(1-2), 1993, pp. 77-83
Being able to obtain accurate estimates for the number of Haemonchus c
ontortus third-stage larvae (L3) on pasture is essential to any type o
f strategy intended for control. The objective of this experiment was
to study the effect of the time, from collection to processing, on the
recovery rate of H. contortus L3 from herbage. Separate herbage sampl
es were inoculated with two treatments of a known amount of larvae on
Day 1 and then samples were processed from Day 1 to Day 30. This simul
ated infective herbage was collected, refrigerated, and processed over
a period of 1 month. A drop in the recovery rate over time was found.
Treatment 2 (5000 L3) exhibited a sharper decline in recovery rate th
an Treatment 1 (1800 L3). These findings suggest that the effect of ti
me, from collection to processing, as well as larval concentration on
the herbage, must be considered when performing any type of larval rec
overy technique.