Cc. Lin et al., PICOGRAM DETERMINATION OF AN AVERMECTIN ANALOG IN DOG PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION, Journal of liquid chromatography & related technologies, 20(3), 1997, pp. 443-458
Citations number
17
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A sensitive and automated method for the determination of a new averme
ctin analog (MK-324) in dog plasma has been developed and validated. T
he drug and internal standard were extracted from plasma via solid-pha
se extraction using an automated Gilson ASPEC XL system. The extracts
were evaporated to dryness and reconstituted in a mixture of N,N-dieth
ylmethylamine and acetonitrile. Automated pre-column derivatization of
the reconstituted extracts using trifluoroacetic anhydride was perfor
med prior to injection onto the HPLC system. The fluorescent derivativ
es of the drug and internal standard were chromatographed using a Zorb
ax RX-C18 column and a mobile phase composed of acetonitrile, water an
d tetrahydrofuran [6:1:1 (v/v/v)]. The assay was validated in the conc
entration range of 100 - 5,000 pg/mL. Its applicability, inter-day per
formance and specificity were demonstrated by analyzing plasma samples
from six studies with MK-324.