1. Ileal endogenous amino acid losses were determined in broiler chick
ens and in cannulated cross-bred layer strain cockerels using either a
nitrogen-free diet, regression analysis or a 48 h fast. 2. Endogenous
amino acid flows to the ileum in fasted cockerels were significantly
lower than those obtained both by feeding the nitrogen-free diet, and
from regression analysis in either broilers or cockerels. Regression a
nalysis gave the highest flows. 3. The apparent digestibility coeffici
ents of amino acids in a diet containing 200 g/kg crude protein were l
ower in broilers (0.84) than in cockerels (0.88). When corrected, by r
egression analysis, for the contribution of endogenous amino acids, th
e true digestibility coefficients became 0.90 and 0.92 respectively.